Global transcriptomic profiling demonstrates induction of oxidative stress and of compensatory cellular stress responses in brown trout exposed to glyphosate and Roundup

Table 1 ERCC spike-in control analysis for assessment of transcript expression reproducibility and dynamic range in all individual samples sequenced

Sample	R ²	Log2 dynamic range (FPKM)	Sample	R ²	Log2 dynamic range (FPKM)	Sample	R ²	Log2 dynamic range (FPKM)
C1	0.93	13.74	LG1	0.92	14.36	LR1	0.93	14.91
C2	0.91	14.52	LG2	0.91	14.52	LR2	0.91	14.64
C3	0.92	14.54	LG3	0.91	15.16	LR3	0.92	13.71
C4	0.94	15.61	LG4	0.91	15.32	LR4	0.93	13.32
C5	0.94	14.21	LG5	0.92	13.92	LR5	0.91	14.53
C6	0.94	14.29	LG6	0.90	14.49	LR6	0.93	16.05
C7	0.92	13.88	HG1	0.90	14.18	MR1	0.91	14.00
C8	0.92	13.83	HG2	0.91	15.26	MR2	0.93	13.97
C9	0.89	14.11	HG3	0.93	15.51	MR3	0.91	15.60
C10	0.92	14.28	HG4	0.90	14.99	MR4	0.92	14.07
C11	0.91	16.19	HG5	0.90	14.92	MR5	0.93	13.86
C12	0.93	15.26	HG6	0.93	14.76	MR6	0.92	15.70
						HR1	0.91	16.08
						HR2	0.90	13.95
						HR3	0.92	14.08
						HR4	0.91	14.08
						HR5	0.93	15.75
						HR6	0.92	15.19

1. R² values represent the correlation between measured FPKM values and expected concentrations of control transcripts.
2. Dynamic range was calculated from maximum-minimum FPKM values of control transcripts for all individual libraries, using only control transcripts that had ≥1 mapped read in ≥6 replicate samples.

ISSN: 1471-2164