Figure 4

Novel transcripts are found in leishmania exosomes. A. Artemis genome browser alignments of the L. donovani exosome library and a L. donovani spliced leader sequence library (P. Myler, unpublished) with the L. donovani reference genome. Shown are two regions with reads mapping to them. Top: intergenic region on chromosome 30 (LdBPK_301180_leftof), bottom: intergenic region on chromosome 29 (LdBPK_291610_leftof). Light blue boxes on grey tracks are annotated genes. In dark blue are the reads from the spliced leader library, in red the reads from the exosome libraries. “P” designates the regions that were used for designing probes for Northern blotting. B. Northern blots with probes designed for novel transcripts found in exosomes, corresponding to the genomic regions shown in panel A (301180_leftof and 291610_leftof), plus an additional probe against a 5.8S rRNA (27rRNA5). L. donovani total (T) and exosome (E) RNA were probed on the same membrane. Equal amounts of RNA (3 μg) were loaded in each lane. Sizes of bands on membranes as indicated are in nucleotides (nt) and were calculated based on 262 nt, 150 nt and 21 nt size markers.