Table 4 Characteristics of the novel microsatellite marker system and the genetic diversity of Chengdu captive giant panda population, including locus names, primer sequences, accession number, repeat unit, fluorescent dyes, annealing temperatures (Tm), length (bp), numbers of individuals genotyped (N), numbers of alleles(k), observed heterzygosity (HObs), expected heterzygosity (HExp), allelic richness (A R ), Polymorphism Information Contents (PIC), HWE P values (P-value)
Locus name | Primer sequences(5′—3′) | Accession no. | Repeat unit | Fluorescent dyes | Tm (°C) | Length (bp) | N | k | HObs | HExp | A R | PIC | P-value |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
gpz-6 | F: CCTGGCAGGGCAAAGTATT | KF907161 | (AAAG)11 | FAM | 60 | 202 | 56 | 6 | 0.714 | 0.689 | 6.000 | 0.633 | 0.2615 |
R: CCCCGTGAAAACATCAAGAC | |||||||||||||
gpz-47 | F: GACCTCAGTGTACGCCCAGT | KF907176 | (AATG)20 | TAMRA | 60 | 230 | 57 | 4 | 0.544 | 0.524 | 4.000 | 0.468 | 0.3557 |
R: CTGGACAGGCAGGTAGAAGC | |||||||||||||
gpz-20 | F: CCCTCTCGTTGTGTCTCTCTG | KF907169 | (AAAG)10 | FAM | 63 | 248 | 52 | 10 | 0.731 | 0.724 | 10.000 | 0.695 | 0.1302 |
R: CACCTGGTAAATGGCACCTT | |||||||||||||
GPL-47 | F: TCCCCCTCTATGGTAAAAGG | KF907147 | (TCTA)20 | FAM | 65 | 180 | 53 | 6 | 0.849 | 0.819 | 6.000 | 0.783 | 0.2161 |
R: CCATGTTGGGTGTAGGGATT | |||||||||||||
GPL-29 | F: TCCAAGGCTTCAAACAAGGT | KF907139 | (ATCC)19 | TAMRA | 60 | 215 | 56 | 4 | 0.714 | 0.677 | 4.000 | 0.617 | 0.2800 |
R: CACCACAGGTGCCAATTATG | |||||||||||||
GPL-60 | F: TGCCGGAAAGTTCTAAGCAT | KF907152 | (TCTT)12 | FAM | 63 | 218 | 57 | 5 | 0.702 | 0.719 | 5.000 | 0.668 | 0.3991 |
R: TTTCTCTCCCTCTCCCCTTC | |||||||||||||
gpz-54 | F: CAATATTTTAAGGCGTGGGACT | KF907181 | (AGAT)18 | TAMRA | 63 | 245 | 56 | 5 | 0.714 | 0.704 | 4.929 | 0.643 | 0.6226 |
R: GCATAATTGCAGAACCAGAGC | |||||||||||||
GPL-8 | F: TGGTTTTGCAAGGATGACAG R:TTGTGACAAGCAAGCTCCAC | KF907132 | (ATCC)11 | HEX | 63 | 248 | 54 | 4 | 0.648 | 0.639 | 4.000 | 0.584 | 0.4311 |
GPL-31 | F: GCATCCTTGTCCTCTTGGAG | KF907141 | (ATCT)21 | FAM | 60 | 183 | 57 | 3 | 0.632 | 0.585 | 3.000 | 0.490 | 0.1640 |
R:GCATTGTTTTCTACTCTACAAATATCC | |||||||||||||
GPL-44 | F: TTCTCCCTCTGTCTGCCACT | KF907146 | (ATAA)21 | FAM | 63 | 232 | 53 | 3 | 0.491 | 0.525 | 3.000 | 0.461 | 0.2543 |
R: ACCATTCTGGGTGCGATAAC | |||||||||||||
gpz-51 | F: GGGGAGGATATGTGTTGTGG | KF907179 | (AGAT)11 | TAMRA | 60 | 175 | 57 | 4 | 0.579 | 0.503 | 3.993 | 0.424 | 0.0440 |
R: TGCTTTGGATTTATTGGAGCA | |||||||||||||
GPL-28 | F: GAAAGAAGGGCAGGGATAGG | KF907138 | (ATAA)21 | FAM | 63 | 238 | 56 | 3 | 0.536 | 0.486 | 2.995 | 0.382 | 0.2594 |
R: TGACCAAGAACTCACGGTTG | |||||||||||||
GPL-53 | F: CCAGAAAATGGCTTTCATGC | KF907148 | (ATTT)21 | HEX | 65 | 210 | 55 | 6 | 0.382 | 0.380 | 5.997 | 0.362 | 0.6395 |
R: TCTCTTTCTCTGCCCCACAC | |||||||||||||
gpy-20 | F: GCAGGCACTCAAGAGGTGTT | KF907159 | (TTTG)16 | TAMRA | 63 | 197 | 56 | 3 | 0.482 | 0.492 | 3.000 | 0.439 | 0.8984 |
R: CCTTGTGCTAAACACAGGTGA | |||||||||||||
gpy-5 | F: CTCGGGAGCTTTGTACCATC | KF907157 | (AACT)16 | HEX | 63 | 228 | 57 | 4 | 0.509 | 0.510 | 3.993 | 0.459 | 0.4341 |
R: CAGAGAGCCCAAACCTCAAC | |||||||||||||
Mean | Â | Â | Â | Â | Â | Â | Â | 4.7 | 0.615 | 0.598 | 4.660 | 0.541 | --- |