Table 1 Output statistics of sequencing and assembly

Reads

Number of reads

26,577,780

27,355,558

33,619,376

Read size (nt/read)

90

90

75

Total nucleotides (nt)

2,392,000,200

2,462,000,220

2,521,453,200

Q20 percentage (%)

89.46

89.66

86.08

N percentage (%)

0.01

0.00

0.00

GC percentage (%)

58.19

57.92

57.00

Contigs

Number of contigs

165,480

142,072

44,112

Mean size of contigs

178

191

386

Length of all contigs (nt)

29,423,650

27,169,112

17,023,492

Scaffolds

Number of scaffolds

35,516

35,448

21,757

Mean size of scaffolds

524

520

776

Length of all scaffolds (nt)

18,593,527

18,426,448

16,891,810

Unigenes

Number of unigenes

25,511

25,214

16,245

20,822

Mean size of unigenes

681

682

994

1,013

Length of all unigenes

17,383,818

17,203,586

16,152,293

21,085,467