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Table 2 Summary of the results from knock down of LsRXR

From: Characterization of a novel RXR receptor in the salmon louse (Lepeophtheirus salmonis, Copepoda) regulating growth and female reproduction

Group

# female on fish at harvest

Length of egg strings (mm)

Hatching of egg (%)

# copepodites

Dead/deformed larvae (%)

Rel. expression of LsRXR (Q-PCR)

control

38

16.8

80.5

412

1.4

1

SD = 1.7, n = 25

SD = 12.8, n = 25

SD = 75, n = 18

SD = 1.8, n = 25

SD = 0.27, n = 18

RNAiFr1

38

8.8

13.3

5

84.5

0.37

SD = 2.7, n = 35

SD = 20.5, n = 25

SD = 10, n = 20

SD = 24.9, n = 13

SD = 0.13, n = 29

RNAiFr2

39

8.3

9.1

1

85.0

0.47

SD = 3.4, n = 24

SD = 8.6, n = 15

SD = 4, n = 15

SD = 29.1, n = 11

SD = 0.15, n = 19

  1. Female lice were counted at the time point of harvesting. These are the remaining ones from the 50 female lice put on 6 fish in each tub after injection of double stranded RNA. Control group lice were injected with double stranded cod RNA (trypsin), RNAi Fr1 and Fr2 group lice were injected with double-stranded RNA fragment one or two (see Figure 1) respectively. The length of egg strings was measured at harvesting and the number of copepodids was reported at day nine after hatching. Photographs of harvested adult females and their egg strings are shown in Additional file 2: Figure S2. The two RNAi groups did not differ significantly in all measured parameters (all t-tests: p > 0.05) while significant differences between control group and the RNAi groups were evident in all measured parameters except number of female lice on fish (T-tests: length of egg strings: p < 1.98*10−25, hatching success p < 2.54*10−25, number of copepodids: p < 2.2*10−14, proportion of dead/deformed larvae. p < 9.7*10-14, relative expression: P < .1.7*10−7).