Effects of testosterone on liver histology and hepatic lipids. (A) Liver sections stained with hematoxylin and eosin. Micro- and macrovesicular steatosis are indicated by arrow heads, and inflammation is indicated by arrows. (B) Oil red O staining of fat in hepatocytes. Increased lipid deposition is indicated by arrows. (C) Hepatic triglyceride (TG) content in IM, CM, and CMT pigs. (D) Hepatic cholesterol (TC) content in IM, CM, and CMT pigs. IM: intact male pigs fed an HFC diet; CM: castrated male pigs fed an HFC diet; CMT: castrated male pigs fed an HFC diet and given testosterone replacement therapy. Data are expressed as means ± SEMs, n = 6 per group. *P < 0.05; **P < 0.01.