RNA-Seq analyses of differentially expressed genes (DEGs) in livers of IM, CM, and CMT pigs. (A) The numbers of upregulated and downregulated DEGs between groups. (B) Venn diagram showing the overlap of DEGs between groups. (C) Heat map for hierarchical cluster analysis of DEGs between samples. Red: upregulated genes; yellow: downregulated genes. IM: intact male pigs fed an HFC diet; CM: castrated male pigs fed an HFC diet; CMT: castrated male pigs fed an HFC diet and given testosterone replacement therapy.