Figure 7
From: Analysis of the genetic diversity of influenza A viruses using next-generation DNA sequencing
RT-PCR amplification of influenza A virus PR8 and PR8mut genomic RNA. (A) Electrophoretic analysis of RT-PCR products of PR8 and PR8mut separated on a 1.5% agarose gel and subsequently stained with Ethidium Bromide. PB1: polymerase basic 1, PB2: polymerase basic 2, PA: polymerase acidic, HA: hemagglutinin, NP: nucleoprotein, NA: neuraminidase, M: matrix, NS: non-structural. The amplified PB1 and PB2 RT-PCR products run at the same position in the gel. * = aspecific amplification product of 847 bp. (B) Optimized RT-PCR product resolved as in A.