Table 1 Alignment metrics for Illumina MiSeq and Ion Torrent PGM sequencing runs
From: Analysis of the genetic diversity of influenza A viruses using next-generation DNA sequencing
| Â | Illumina MiSeq | Ion Torrent PGM | ||||||
|---|---|---|---|---|---|---|---|---|
| Â | pHW197-M | pHW197-Mmut | pHW197-M | pHW197-Mmut | ||||
| Â | S1 | S2 | S1 | S2 | S1 | S2 | S1 | S2 |
Minimum coverage | 683 | 744 | 815 | 609 | 3532 | 4510 | 3995 | 4830 |
Maximum coverage | 27389 | 28589 | 32802 | 26275 | 15716 | 17632 | 14664 | 18196 |
Average coverage | 15369 | 16315 | 18236 | 14610 | 11525 | 13236 | 11118 | 13636 |
Standard deviation | 6739 | 7120 | 7888 | 6315 | 3323 | 3499 | 2853 | 3562 |
Unmapped reads (%) | 0.20 | 0.16 | 0.21 | 0.22 | 1.06 | 1.05 | 1.28 | 1.19 |
Unmapped bases (%) | 0.17 | 0.14 | 0.19 | 0.19 | 1.07 | 1.05 | 1.26 | 1.18 |