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Table 2 Number of detected variants in the pHW197-M sample before and after filtering

From: Analysis of the genetic diversity of influenza A viruses using next-generation DNA sequencing

 

Illumina MiSeq

Ion Torrent PGM

 

Before

After a

Before

After a

 

S1 b

S2 b

S1 b

S2 b

S1 b

S2 b

S1 b

S2 b

SNP c

4

4

0

0

0

0

0

0

Insertion

0

0

0

0

14

12

3

1

Deletion

0

2

0

1

71

66

3

4

  1. aThe filtering parameters used were average quality threshold > Q20, forward/reverse balance > 0.25, and independent counts of variant > 10.
  2. bSequencing was performed in duplicate (S1 and S2).
  3. cSNP = single nucleotide polymorphism.