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Table 3 Sensitivity of Illumina MiSeq and Ion Torrent PGM

From: Analysis of the genetic diversity of influenza A viruses using next-generation DNA sequencing

  

Illumina MiSeq

Ion Torrent PGM

  

797

1088

797

1088

pHW197-M

pHW197-Mmut

C

T

A

T

C

T

A

T

0

100

< d.l.

99.97

< d.l.

99.96

< d.l.

99.56

< d.l.

99.89

0

100

< d.l.

99.95

< d.l.

99.94

< d.l.

99.62

< d.l.

99.96

95

5

94.84

5.14

95.40

4.58

95.22

4.75

95.02

4.96

99

1

98.78

1.19

98.93

1.06

98.97

1.02

98.96

1.02

99.9

0.1

99.80

0.17

99.85

< d.l.

99.93

< d.l.

99.87

< d.l.

  1. The observed mutation frequencies (%) after mapping the reads to the reference sequence of pHW197-M are shown. < d.l. = mutation frequency falls below detection limit (< 2*error rate, < 0.16% for Illumina MiSeq and < 0.24% for Ion Torrent PGM). The pHW197-Mmut plasmid contains the tracer mutations C797T and A1088T.
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BMC Genomics

ISSN: 1471-2164

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