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Table 6 Sensitivity of Illumina MiSeq and Ion Torrent PGM to detect mutations in viral samples

From: Analysis of the genetic diversity of influenza A viruses using next-generation DNA sequencing

 

Illumina MiSeq

Ion Torrent PGM

 

354

645

354

645

PR8

PR8mut

C

T

A

T

C

T

A

T

0

100

< 0.5

99.96

< 0.5

99.95

< 0.5

99.61

< 0.5

99.95

0

100

< 0.5

99.96

< 0.5

99.95

< 0.5

99.62

< 0.5

99.95

99

1

98.21

1.77

98.31

1.64

98.90

0.76

98.95

0.90

  1. The observed mutation frequencies (%) after mapping the reads of the PR8 and PR8mut viral samples to the wild type PR8 viral reference genome (based on the sequence from the plasmids from which the virus was produced) are shown. The PR8mut virus contains the tracer mutations C354T and A645T.
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BMC Genomics

ISSN: 1471-2164

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