Comparative whole-genome analyses of selection marker–free rice-based cholera toxin B-subunit vaccine lines and wild-type lines

Table 1 Summary of sequence reads for each line

Line	Total reads	Mapped reads	Mapping rate (%)	Genome coverage (bp)	Coverage rate (%)	Depth (fold)
50A	384,384,291	375,891,543	97.8	371,762,264	99.6	101.3
51A	362,591,974	352,782,360	97.3	371,570,800	99.6	95.1
55A	356,620,373	349,808,296	98.1	369,691,614	99.0	94.7
WT1	342,044,404	332,246,878	97.1	371,589,770	99.6	89.4
WT2	323,436,829	314,249,500	97.2	371,575,970	99.6	84.3

Mapping rate represents the ratio of the number of mapped reads to that of total reads. Covered length represents the number of genome bases covered with at least one read. Coverage rate is the ratio of covered length to the total length of the rice reference genome (373,245,519 bps, IRGSP-1.0, build 5 [21]). Depth was calculated by dividing the total length of all mapped reads (100 bps for each read) by covered length.

ISSN: 1471-2164