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Figure 3 | BMC Genomics

Figure 3

From: Inorganic Arsenic-induced cellular transformation is coupled with genome wide changes in chromatin structure, transcriptome and splicing patterns

Figure 3

Arsenite treatment causes a reduction in nucleosome spacing. A) Nuclei from control and arsenic-transformed BEAS-2B cells were treated with MNase and the profile of bulk chromatin was analyzed by gel electrophoresis to calculate the nucleosome repeat length (NRL). B) Plot of nucleosome number versus DNA length for MNase-digested bulk chromatin of BEAS-2B cells ± arsenic. This plot was used to calculate the corresponding NRL. Data from 3 independent experiments has been pooled and the average ± SD is shown. Blue bars and red bars correspond to nucleosome ladders from NT and iAs-T cells respectively. C) Similar studies were done in HeLa cells and D) NRL was calculated in NT, iAs-T and iAs-Rev cells.

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