Group II aGPCR expression data – part 2. Relative expression of zebrafish Group II aGPCRs obtained with either high-throughput quantitative real-time PCR or conventional qPCR in a collection of 12 developmental time-points and 10 adult tissues. (A)
emrl8, and (H)
emrl9. Error bars display the upper (RQmax) and lower (RQmin) limits of possible relative quantification values. Fold changes shown are relative to expression at 21 dpf (denoted by gray line at y = 1). emrl1 and emrl2 could not be distinguished at the nucleotide level with unique primers and so emrl1/emrl2 expression is shown (A).