Figure 3

MiRNA recovery from decreasing amounts of plasma input. The impact of plasma volume on the recovery of (A) exogenous RNA sequences and (B) endogenous miRNAs was evaluated using real-time RT-PCR. Plasma samples were diluted in water to 50 μL and processed with the QIAGEN miRNeasy Mini Kit. In (A), three synthetic sequences of different amounts were spiked into the samples and measured in six technical replicates. Significance compared to the water-only control was determined using a linear mixed effects model, **** = p < 0.0001. Endogenous miRNAs were not detected in any water-only controls (Cq > 40).