Figure 4From: Optimized microRNA purification from TRIzol-treated plasma MiRNA changes in one rhesus macaque following exposure to aerosolized Ebola virus. Plasma samples from a single NHP challenged with aerosolized Ebola virus were processed using the optimized miRNA extraction protocol. Individual miRNAs were quantified using the Exiqon microRNA Ready-to-Use PCR Human panel I + II V3.R. Twenty-five miRNA sequences (colored filled circles above the horizontal line at p = 0.0001) were significantly altered between virus exposure and time of death. Orange circles indicate upregulated miRNAs, while blue circles specify downregulated sequences. A paired t-test using three PCR technical replicates at each timepoint (day 0 and day 7) was used to identify differentially expressed miRNAs in these datasets.Back to article page