Functional analysis of C1 family cysteine peptidases in the larval gut of Тenebrio molitor and Tribolium castaneum

Table 1 Predicted cysteine cathepsin genes (B, L, O, K, and F) in the T. castaneum genome, and relative expression levels in the larval gut, as estimated by transcriptome and microarray data

Protein ID	Gene ID	Tc annotation ¹	Chromosome	Expression (RPKM)	Gut rank ²	Active site residues ³	NCBI annotation
NP_001164001	LOC659441	11001	10	77,228.22	82	QCHN	cathepsin L
NP_001164314	LOC659502	11000	10	25,848.86	14	QCHN	cathepsin L
XP_970644	LOC659226	11003	10	42.77	7	QCHN	cathepsin L
XP_970773*	LOC659367	11002	10	35.06	2	ESHN	cathepsin L homolog
XP_970951	LOC659565	10999	10	0.28	2	QCHN	cathepsin L
XP_971698	LOC660368	09365	7	2387.62	44	QCHN	cathepsin L
XP_971867	LOC660551	09362	7	1.99	1	QCHN	cathepsin L
XP_971752	LOC660428	09364	7	0.98	1	QCHN	cathepsin L
XP_971975	LOC660669	09448	7	0.02	2	QCHN	cathepsin L
NC_007422	LOC660491	pseudogene?	7	-	NOC		-
XP_974298	LOC663145	02952	3	3,142.15	43	QCHN HH	cathepsin B
NP_001164205	LOC663117	02953	3	1,132.96	46	QCHN HH	cathepsin B
XP_974244	LOC663090	02954	3	248.71	9	QCHN HH	cathepsin B
XP_974220	LOC663066	02955	3	79.91	2	QCHN	cathepsin B-like
XP_966750	LOC655148	05431	8	443.27	3	QCHN	cathepsin B-like
XP_966663	LOC655077	05432	8	1.18	3	QCHN	cathepsin B-like
XP_968689*	LOC657117	05954	8	57.60	NOC	QSTN	cathepsin B homolog
XP_968767	LOC657203	05953	8	82.17	3	QCHN	cathepsin B-like
XP_008196467⁴	LOC656957	05955/05956	8	-	NOC	QCHN	cathepsin B-like
XP_008196465⁴	LOC657038	---	8	-	NOC	QCHN	cathepsin B-like
NP_001164088	LOC663234 (26-29-p)	--- (09486)	7	1,309.16	NOC	QCHN	cathepsin L
XP_969833	LOC658343	02843	3	0.02	1	QCHN	cathepsin L
XP_967834* (XP_008195382)⁵	LOC656198	09217	7	28.64	1	QSHN	cathepsin B homolog
XP_970512	LOC659087	07214	4	11.99	NOC	QCHN	cathepsin O
XP_973607 (XP_3195656)⁶	LOC662417	---	7	2.32	NOC	QCHN	cathepsin F
XP_001814509	LOC100141668	13582	1 (X)	0	1	QCHN	cathepsin K

¹From [1]. Tc09363 and Tc01950 were in the original annotation but have been removed from the annotations of cysteine cathepsins; Tc09486 was missed in the original annotation.
²As defined in [21], from microarray gene expression data from larval gut tissue (higher ranks=higher expression); NOC – not on chip.
³Active site residues including those in occluding loop [55].
⁴Changed in the Tcas4 genome build; listed as a pseudogene in Tcas3, and no expression values available.
⁵Now annotated by NCBI as tubulointerstitial nephritis antigen-like.
⁶Changed in Tcas4 genome build.
*Predicted homologs according to lack of sequence conservation in active site residues.

ISSN: 1471-2164