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Table 2 Predicted cysteine cathepsin genes in the T. molitor genome, and relative expression levels in the larval gut, as estimated by transcriptome data

From: Functional analysis of C1 family cysteine peptidases in the larval gut of Тenebrio molitor and Tribolium castaneum

Identification

NCBI accession 1

Previous iterations (% Identity)

Expression (RPKM)

Expression (Rescue)

Active site residues 7

Predicted annotation

TmL13

KP303287

AM4-22 (ABC88769, 99%), AM3-32 (ABC88768, 99%)2; TmCysII, TmCysIII3; ppCal3 (AAP94048)4; 3QT45; Cont-08897, Bt-075835

19,726.5

8,496.6

QCHN

cathepsin L

TmL5

KP303279

ppCAL2 (AAR05023, 97%)4; 3QJ35; Cont-01354, Bt-075286

1,356.6

572.7

QCHN

cathepsin L

TmL11

KP303285

Cont-00009, Bt-014976

1,149.4

354.9

QCHN

cathepsin L

TmL2

KP303276

ppCAL1a,b,c (AAP94046, 100%)4; Cont-09057, Bt-001115

337.3

263.5

QCHN

cathepsin L

TmL4

KP303278

 

326.9

168.9

QCHN

cathepsin L

TmL1

KP303275

 

162.2

113.9

QCHN

cathepsin L

TmL30*

KP303289

 

130.3

104.4

ESHN

cathepsin L homolog

TmL29*

KP303289

 

130.3

104.4

QAHN

cathepsin L homolog

TmL3

KP303277

AAP94047 (91%)3

62.1

31.3

QCHN

cathepsin L

TmL9

KP303283

 

72.0

46.0

QCHN

cathepsin L

TmL7

KP303281

 

25.3

11.7

QCHN

cathepsin L

TmL6

KP303280

Bt-07886

15.8

5.8

QCHN

cathepsin L

TmL8

KP303282

 

5.7

3.7

QCHN

cathepsin L

TmL15

KP303288

 

0.2

0.2

QCHN

cathepsin L

TmB33

KP303302

AM4-18 (ABC88766, 98%)2; TmCysII3; Cont-09310, Bt-002495

2,489.6

1,160.4

QCSN HH

cathepsin B

TmB20

KP303293

АМ3-87 (ABC88767, 99%)2; Cont-008906

448.4

221.5

QCHN

cathepsin B-like

TmB25

KP303297

 

672.6

296.7

QCHN

cathepsin B-like

TmB26

KP303298

Cont-08975, Bt-082376

657.5

431.6

QCHN

cathepsin B-like

TmB18

KP303291

 

283.2

175.8

QCHN HH

cathepsin B

TmB17

KP303290

Cont-00240, Bt-014536

163.9

99.0

QCHN HH

cathepsin B

TmB32*

KP303301

 

77.9

37.5

QSHN

cathepsin B homolog

TmB23

KP303295

 

48.8

29.3

QCHN

cathepsin B-like

TmB19

KP303292

 

34.2

24.9

QCHN

cathepsin B-like

TmB27

KP303299

 

26.5

13.9

QCHN

cathepsin B-like

TmB24

KP303296

 

20.0

6.5

QCHN

cathepsin B-like

TmB28

KP303300

 

4.0

2.2

QCHN

cathepsin B-like

TmB22

KP303294

 

1.2

0.7

QCHN

cathepsin B-like

TmO12

KP303286

 

38.4

22.2

QCHN

cathepsin O

TmF10

KP303284

 

23.4

8.4

QCHN

cathepsin F

  1. 1Accession numbers are for predicted mRNA.
  2. 2[16].
  3. 3[14,18].
  4. 4[19].
  5. 5[41].
  6. 6[27].
  7. 7Active site residues including those in occluding loop [55].
  8. *Predicted homologs according to lack of sequence conservation in active site residues.