Table 4 Comparison of putative cathepsin B orthologs in T. molitor and T. castaneum , and comparison of key residues to those in human cathepsin B (P07858)
Substrate binding site | Substrate binding site | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Key residues 1 | Key residues | ||||||||||
S1 | S2 | S1 | S2 | ||||||||
23|65 | 67|68 | 133 | 157 | 205 | 23|65 | 67|68 | 133 | 157 | 205 | ||
Cathepsin B human | GG | YP | A | G | E | GG | YP | A | G | E | |
Tenebrio molitor | Tribolium castaneum | ||||||||||
TmB33 | GG | WP | D | G | D | XP_974298 | GG | WP | D | G | D |
TmB18 | GG | YP | S | G | D | NP_001164205 | GG | MP | S | G | G |
TmB17 | GG | FP | A | G | E | XP_974220 | GG | FP | A | G | S |
TmB20 | GG | YM | N | G | Y | XP_974244 | GG | YM | S | G | N |
TmB19 | GG | YI | G | G | Y | ||||||
TmB22 | GG | YM | G | G | N | ||||||
TmB23 | GG | YV | T | G | Y | ||||||
TmB24 | GG | AP | N | G | N | XP_966663 | GG | YS | S | G | N |
TmB25 | GG | WP | S | G | N | XP_966750 | GG | AP | H | G | Y |
TmB27 | GG | WM | A | F | Q | ||||||
TmB26 | GG | SS | S | G | N | ||||||
XP_008196467 | GG | YO | Y | G | E | ||||||
XP_008196465 | GG | YT | T | X | E | ||||||
XP_968767 | GG | YS | G | G | S | ||||||
XP_968689* | SG | YT | A | G | S | ||||||
TmB28* | SG | SS | I | S | H | ||||||
TmB32* | GG | YL | T | G | F | XP_008195382* | GG | YL | T | G | F |