Figure 5

The regulatory modes of NdgR. (A) Measurement of expression levels of NdgR target genes in the sulfate assimilation pathway in various combinations of input signals. D, N and T denote diamide treatment, ndgR gene and target genes of NdgR, respectively. D = 0 or 1 indicates nontreatment or treatment of diamide, respectively. The absence or presence of the ndgR gene is denoted as 0 or 1, respectively. Expression of target genes above threshold are denoted as 0 (OFF) or 1 (ON) of output signals. Expression levels were normalized relative to the expression levels of controls (D = 0, N = 0). All the values are represented with standard deviations. (B) The logic gates of NdgR regulatory networks. NdgR regulates the sulfate assimilation pathway using coherent and incoherent FFL.