Location relationship of lcp1 and lcp2 with chromosome CI. (A) Schematic diagram of the two putative locations of lcp1 and lcp2 on chromosome CI based on sequence information. Identical sequences in chromosome CI and the plasmids are shown in gray shadow. The locations of the primers used for PCR amplification are indicated (“C” indicates chromosome, “P” indicates plasmid). The figure is drawn to scale. (B) PCR results confirming the plasmids are circular replicons outside of the chromosome CI. PCR amplification was performed with the primers indicated.