Phylogenetic tree of CYP450s. Phylogenetic tree of the P. vienamensis var. fuscidiscus CYP450s. Phylogenetic tree constructed based on the deduced amino acid sequences for the P. vienamensis var. fuscidiscus CYP450s (bold letters) and other plant CYP450s involved in triterpenoid biosynthesis. Protein sequences were retrieved from NCBI GenBank using the following accession numbers: Vitis vinifera VvCYP716A15, (BAJ84106.1) and VvCYP716A17 (BAJ84107.1); Medicago truncatula MtCYP716A12, (ABC59076.1), MtCYP93E2 (ABC59085), MtCYP72A63 (H1A981.1), MtCYP72A65v2, (BAL45202), MtCYP72A67v2 (BAL45203) and MtCYP72A68v2 (BAL45204), and MtCYP72A61v2 (BAL45199); Panax ginseng PgCYP716A52v2 (AFO63032.1), PgCYP716A53v2 (I7CT85.1) and PgCYP716A47 (H2DH16.2); Arabidopsis thaliana AtCYP708A2 (NP_001078732.1) and AtCYP705A5 (EFH40098); Glycyrrhiza uralensis GuCYP88D6 (B5BSX1.1), GuCYP93E3 (BAG68930) and GuCYP72A154 (H1A988.1); Avena strigosa AsCYP51H10 (ABG88965.1); Glycine max GmCYP93E1 (NP_001236154.1).