Confirmation of differential miRNA expression. To confirm the differential expression of miR-30a, miR-143, miR-191*, and miR-223 in SAH and sham animals as well as the lack of differential expression of miR-145, additional qPCR assays were performed. Fold changes over sham were calculated using the 2–ΔΔCt quantitative method and analyzed with Student’s t-tests. The differential expression of miR-191* and miR-223 could not be confirmed. Fold change over sham for miR-30a (A), miR-143 (B), miR-191* (C), miR-223 (D), and miR-145 (E) at 0 h, 1 h, 6 h, and 24 h post-SAH. Sham (white bars) is set to 1. * = p < 0.05, ** = p < 0.01, compared to sham.