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Table 2 Summary of amplicon sizes, mutation frequency, and primers used for the amplification

From: TILLING by sequencing to identify induced mutations in stress resistance genes of peanut (Arachis hypogaea)

Gene

Amplicon (bp)

F primer no.

Sequence (5'-3')

R primer no.

Sequence (5'-3')

Ara h 1.01

1,865

1306

GAGCAATGAGAGGGAGGGTT

2079

TCTTCGTCTTCGTCCTCCTCTTCTT

Ara h 1.02

1,666

1306

GAGCAATGAGAGGGAGGGTT

1309

CCTCCTCTTCTTCCCACTCTTG

Ara h 2.01

1,300

815

CGATTTACTCATGTACAATTAACAATAGAT

817

TCAAGATGGTTACAACTCTTGCAGCAACA

Ara h 2.02

1,247

816

ATCACCTTAAATTTATACATATTTTCGG

817

TCAAGATGGTTACAACTCTTGCAGCAACA

Ah FAD2A

1,241

1048

CTCTGACTATGCATCAG

1055

GATTACTGATTATTGACTT

Ah FAD2B

1,234

1048

CTCTGACTATGCATCAG

1101

CAGAACCATTAGCTTTG

AhLOX7_5'

1,713 1,714 1,715 1,716

2199

GCAGGAGAAGCAGCATTCACAGTTA

2184

CTCAAGAGGAACATTATCCC

AhLOX7_3'

1,532 1,544 1,698 1,710

2186

AAAGTCTACGGTGATCAAACCAGC

2188

AGCAGACACACCCATTGAAA

AhPLD1

1,271 1,272

2120

GACTTACGAACCTCAAAGATGCTGG

2121

TACTCTCCGTCCTTCTTCGCTT

AhPLD2

1,500

1991

AAGAACTGGGCACGTGGTGTTAGGAGT

1992

TCGACGGTTCTCCTGGGCTTTTATGTA