Table 3 Mutation prediction parameters in various ranges for single copy genes
Min quality | Minimum read percentage with non-reference nucleotide 1 (%) | Background read percentage with non-reference nucleotide 1 (%) | Maximum read percentage with non-reference nucleotide (%) | Row non-reference % multiplier | All mutants (uniquely found 2 ) | Known mutants (uniquely found 2 ) | No. of new mutations validated |
|---|---|---|---|---|---|---|---|
10 | 0.370 | 0.033 | 5.000 | 0.67 | 33 | 6 | 9 |
12 | 0.370 | 0.030 | 5.000 | 0.67 | 34 | 6 | 8 |
14 | 0.370 | 0.030 | 5.000 | 0.67 | 34 | 6 | 8 |
15 | 0.370 | 0.029 | 5.000 | 0.67 | 32 | 6 | 8 |
16 | 0.360 | 0.029 | 5.000 | 0.67 | 31 | 6 | 9 |
17 | 0.190 | 0.026 | 5.000 | 0.67 | 55 | 6 | 12 |
18 | 0.170 | 0.023 | 5.000 | 0.67 | 54 | 6 | 13 |
19 | 0.170 | 0.020 | 5.000 | 0.67 | 52 | 6 | 13 |
20 | 0.135 | 0.018 | 5.000 | 0.67 | 71 | 6 | 12 |
21 | 0.058 | 0.017 | 5.000 | 0.67 | 355 | 3 | 7 |