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Table 5 The top ten mutant prediction parameters that produced the highest percent of true mutant and false mutant percentage difference using the known mutants as control

From: TILLING by sequencing to identify induced mutations in stress resistance genes of peanut (Arachis hypogaea)

Minimum quality score

Minimum variance multiplier

Minimum variance percentage

Number of true mutants 1

True %

Number of false mutants

False %

Total mutants predicted

Difference

19

12

0.05

11

68.8

1

6.2

16

62.6

19

14

0.05

10

66.7

1

6.7

15

60.0

20

12

0.05

12

63.2

1

5.3

19

57.9

21

12

0.05

12

63.2

1

5.3

19

57.9

16

10

0.05

11

68.8

2

12.5

16

56.3

22

10

0.05

11

61.1

1

5.6

18

55.5

23

8

0.05

11

61.1

1

5.6

18

55.5

21

10

0.05

12

60

1

5

20

55.0

15

10

0.05

10

66.7

2

13.3

15

53.4

16

12

0.05

10

66.7

2

13.3

15

53.4

  1. 1Based on the validated mutants and the known mutants detected from previous study [40].