Figure 4

Gene Ontology treemap based on enrichment of most highly expressed transcripts. Assembly contigs that aligned to ≥80% of a reference D. rerio protein were ranked by FPKM, and the GO classification for the corresponding D. rerio gene were used to determine enrichment of transcripts at the top of the list using GOrilla based on the minimum hypergeometric scoring method. GOrilla analysis showed significantly enriched GO categories that were found on average in the top 9.6% of genes (±4.8% S.D.) using minimum hypergeometric scoring. Redundant GO terms were filtered with the GO Trimming tool and then visualized with REViGO. The box size correlates to the –log10 p-value of the GO-term enrichment. Boxes with the same color are grouped by semantic similarity (SimRel, similarity = 0.7).