Proteins identified by GO-slim categories and identification of splice junction peptides.
A, B. Percentage of proteins identified from (FDR < 1%, ORF database derived from TransDecoder with Pfam and any length ORF) in each GO-slim category based on number of assignable D. rerio genes. C. Region of A. leptorhynchus spectrin alpha 2 that includes alternatively spliced exon containing sequence TAVTKETCSVSVRMKQVEEL. Periods c-terminal to K and R indicate trypsin cleavage sites. Identified peptide sequences are red and bolded. Colored boxes emphasize exons. D, E. Annotated MS/MS spectra of splice junction peptides QDQIENQTAVTK and QDQIENQYGTKKEKGEK.