The central nervous system transcriptome of the weakly electric brown ghost knifefish (Apteronotus leptorhynchus): de novo assembly, annotation, and proteomics validation

Table 3 Summary of annotated transcripts/contigs by RNA types

Categories	# Transcripts (including contig isoforms)	# Contigs
Protein sequences
Sense BlastX (D. rerio)	748,404	40,451
Sense BlastX (Actinopterygii)	759,947	42,459
rRNA
rRNA (identified by RNAmmer)	59	4
rRNA (from D. rerio reference)	2	2
Mitochondrial rRNA (from D. rerio reference)	4	2
Other ncRNA*
snoRNA	211	17
miRNA	340	84
snRNA	116	12
lincRNA	419	54
SRP RNA	1	1
Antisense	75	27
lncRNA (Kaushik et al.)	111	15

Note that some transcripts were found to align to multiple categories, such as the case of transcripts containing protein-coding ORFs as well as a pri-miRNA sequence. *ncRNA was identified from D. rerio reference (ftp://ftp.ensembl.org/pub/release-75/fasta/danio_rerio/ncrna/Danio_rerio.Zv9.75.ncrna.fa.gz) except for rRNA identified with RNAmmer and lncRNA from [93].

ISSN: 1471-2164