Computational simulations of the RNA half-lives as a determinant for transcript level. (a) Computational simulation into the effect of RNA half-life on RNA abundance. Each gene was simulated to have a half-life of 10.9 hours, potential default half-life, and the predicted gene expression was plotted against the H3K4me3 intensities, along with the measured gene expression. The dotted line indicates the least-squares regression line between the predicted gene expression and H3K4me3 intensities. (b-d) Result of the computational simulation of the predicted RNA expression when each RNA abundance was predicted from RNA half-life when (b)UPF1, (c) EXOSC5 and (d) STAU1 were knocked-down, in comparison to each control knock-down conditions. The predicted gene-expression of the decay factor knock-down and the measured gene-expression of control knock-down were plotted against H3K4me3 intensities. The dotted line indicates the least-squares regression line between the predicted gene expression and H3K4me3 intensities.