Table 4 Primers of four defense genes
From: Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer
Genes | Expressed level | Primers for qRT-PCR | Primers for expression vectors |
|---|---|---|---|
Actin | - | S- 5′ CGGCAGCCTCCATACCAA 3′ | - |
A - 5′ GCCAAGAACAGCTCCTCA 3′ | - | ||
bgl | Up | S- 5′ TCGCCACAAAGCAGTAAGC 3′ | S- 5′gcGAGCTCATGGCTCCACTTCTCGCCGCAG3′ |
A- 5′ ACCAAAGATGAAGTCAGAGGG 3′ | A - 5′cgCTCGAGAGCTGGCGTAATAATCTTCTTG3′ | ||
TRXM | Up | S- 5′ TTGGTGATGGCGTGCGAGAC 3′ | S- 5′gcGGATCCATGGCCATGGAGACGTGCTT3′ |
A- 5′ TGGATGCCGTAGGCGTCGT 3′ | A - 5′cgAAGCTTTGAGCTACCGATGTACTTGT3′ | ||
PR1 | Up | S- 5′ TCGCACATCAAGGTGGAGC 3′ | S- 5′gcGGATCCATGGCCTCCGTCAACAGCT3′ |
A- 5′ ATGGTTTAGTTGTAGGCGTCGG 3′ | A - 5′cgAAGCTTGTTGTAGGCGTCGGGGTT3′ | ||
RAB15 | Down | S- 5′ GAGAATGCCTTCGCCTCCTAC 3′ | S- 5′ gcGGATCCATGGCGGCGGCTGATGTGGAGT3′ |
A- 5′ GTCGAGCTCCTTGCCGTT 3′ | A - 5′cgAAGCTTGTCCCTCCAGCCGCCGCCG3′ |