ST40 genome comparison against the D32 reference genome. Generated by BRIG (http://brig.sourceforge.net/ [last access 16.07.2014] ), the circular map illustrates the whole genome comparison of D32 against the other 14 sequenced ST40 isolates and the probiotic isolate Symbioflor 1 Clone DSM 16431. The outer cycle (dark grey) represents the complete genome of the reference strain D32. The shade of color is geared to similarities in origin of the strains (green: isolate from bovine mastitis; blue: animal and human commensals; violet: isolates from human infections; red: human blood culture isolates; turquoise: strain Symbiolfor 1). The inner cycle illustrates the GC content of D32. Location of the PAI is illustrated by a blue colored box, while the red box indicates the presence of an uncharacterized and large genomic island (GI; 138 kb). Additionally, black labels highlighted four identified prophages of D32; A, animal; B, blood culture; C, colonizer; E, endocarditis; H, human; M, bovine mastitis; U, urine.