Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40

BMC Genomics

Table 5 Quality report of hybrid assemblies considering of 454 and Solexa sequencing data

Isolate	No. of assembled reads	Coverage (454) [n-fold]	Coverage(Solexa) [n-fold]	No. scaffolds	Genome size [bp]	GC content [%]
UW6149	5,060,490	12.78	166.36	72	3,011,563	37.46
UW2860	5,318,329	14.68	176.92	74	3,003,615	37.49
UW6724	1,232,615	9.91	39.94	38	3,085,224	37.33
UW7761	2,025,698	12.71	81.94	26	2,939,826	37.53
UW7777	1,504,959	24.05	45.28	24	2,999,678	37.43
UW7780	1,108,686	10.91	37.28	38	3,115,640	37.31
UW7753	1,424,574	11.14	39.48	67	3,119,011	37.09
UW1833	3,127,985	14.89	100.92	66	3,243,986	37.05
UW7779	1,412,336	18.92	37.1	45	3,070,536	37.20
UW7729	972,823	9.01	28.64	75	3,094,285	37.19
UW7801	1,478,988	10.79	56.72	26	2,933,628	37.52
UW6727	1,442,566	12.5	45.58	34	3,330,760	36.91
UW7709	2,403,224	12.98	90.18	36	2,928,951	37.30
UW7742	3,271,151	18.06	101.23	64	3,054,136	37.17

Genomes of 14 selected ST40 isolates were sequenced by using Solexa technology. Reads of 454 and Solexa sequencing were hybrid assembled by using Mira assembler software and resulted in an increased coverage in combination with a reduction of the number of large contigs.

ISSN: 1471-2164