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Table 5 Quality report of hybrid assemblies considering of 454 and Solexa sequencing data

From: Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40

Isolate No. of assembled reads Coverage (454) [n-fold] Coverage(Solexa) [n-fold] No. scaffolds Genome size [bp] GC content [%]
UW6149 5,060,490 12.78 166.36 72 3,011,563 37.46
UW2860 5,318,329 14.68 176.92 74 3,003,615 37.49
UW6724 1,232,615 9.91 39.94 38 3,085,224 37.33
UW7761 2,025,698 12.71 81.94 26 2,939,826 37.53
UW7777 1,504,959 24.05 45.28 24 2,999,678 37.43
UW7780 1,108,686 10.91 37.28 38 3,115,640 37.31
UW7753 1,424,574 11.14 39.48 67 3,119,011 37.09
UW1833 3,127,985 14.89 100.92 66 3,243,986 37.05
UW7779 1,412,336 18.92 37.1 45 3,070,536 37.20
UW7729 972,823 9.01 28.64 75 3,094,285 37.19
UW7801 1,478,988 10.79 56.72 26 2,933,628 37.52
UW6727 1,442,566 12.5 45.58 34 3,330,760 36.91
UW7709 2,403,224 12.98 90.18 36 2,928,951 37.30
UW7742 3,271,151 18.06 101.23 64 3,054,136 37.17
  1. Genomes of 14 selected ST40 isolates were sequenced by using Solexa technology. Reads of 454 and Solexa sequencing were hybrid assembled by using Mira assembler software and resulted in an increased coverage in combination with a reduction of the number of large contigs.