Figure 2

PacBio sequencing of a 6 Kb insert capture library. A) Post-capture library QC. A 6Kb insert capture library was constructed using the custom SMS/PTLS probe set and quality of the post-capture LM-PCR product was analyzed by Agilent 2100 Bioanalyzer. B) Target capture performance. Critical capture sequencing metrics (alignment rate, on-target/buffer rate and base coverage) were calculated after data analysis. C) Read length performance. Sequencing the 6 Kb insert library by PacBio C3/P5 chemistry with a 180-min movie generated a set of read length metrics for post-filter reads and subreads.