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Table 2 Pilot studies of PacBio-LITS libraries

From: PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations

Sample ID

Ins. size

Probe (target size)

Loading /Chemistry/ Movie time (mins)

Total mapped subreads*

Total mapped SRB (Mb)*

Mean mapped SRL (bp)*

Subreads on target (%)*

Avg. Cov. (x)*

≥1x Cov. (%)*

≥20x Cov. (%)*

BAB1123

1 Kb

SMS/PTLS (7 Mb)

Regular/ C2-P2/1 × 90

360,145

277

770

65

20

89

41

BAB1123

4 Kb

SMS/PTLS (7 Mb)

MagBead/ C2-P2/1 × 120

162,600

353

2,169

69

29

90

54

NA12878

4 Kb

MHC (5 Mb)

MagBead/ C2-XL/1 × 120

214,130

409

1,885

50

44

96

70

HS1011

4 Kb

MHC (5 Mb)

MagBead/ C2-XL/1 × 120

329,251

698

2,101

49

73

97

87

NA12878

6 Kb

SMS/PTLS (7 Mb)

MagBead/ C3-P5/1 × 180

395,342

933

2,358

73

91

95

89

  1. *Detailed sequencing metrics showed in Additional file 3: Table S1.; SRB: subread base; SRL: subread length.