Skip to main content

Table 2 Pilot studies of PacBio-LITS libraries

From: PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations

Sample ID Ins. size Probe (target size) Loading /Chemistry/ Movie time (mins) Total mapped subreads* Total mapped SRB (Mb)* Mean mapped SRL (bp)* Subreads on target (%)* Avg. Cov. (x)* ≥1x Cov. (%)* ≥20x Cov. (%)*
BAB1123 1 Kb SMS/PTLS (7 Mb) Regular/ C2-P2/1 × 90 360,145 277 770 65 20 89 41
BAB1123 4 Kb SMS/PTLS (7 Mb) MagBead/ C2-P2/1 × 120 162,600 353 2,169 69 29 90 54
NA12878 4 Kb MHC (5 Mb) MagBead/ C2-XL/1 × 120 214,130 409 1,885 50 44 96 70
HS1011 4 Kb MHC (5 Mb) MagBead/ C2-XL/1 × 120 329,251 698 2,101 49 73 97 87
NA12878 6 Kb SMS/PTLS (7 Mb) MagBead/ C3-P5/1 × 180 395,342 933 2,358 73 91 95 89
  1. *Detailed sequencing metrics showed in Additional file 3: Table S1.; SRB: subread base; SRL: subread length.
\