Figure 2

Expression levels of olfactory genes in male and female S. litura antennae measured in single-end RNA-Seq (A) and RT-qPCR (B). In single-end RNA-Seq, expression was calculated with log scale of RPKM value. The significant difference between female and male was justified by method of Audic and Claverie (1997) and indicated by symbol “*” (FDR < 0.01 and P < 0.05). In RT-qPCR, gene expression was calculated by the 2^-∆∆Cq algorithm with male as control, GAPDH and UCCR as reference genes. Female gene expression is presented normalized to male antennal expression arbitrarily defined as 1. Error bars signify SD. Significance of difference between male and female responses indicated by *P < 0.05, “#” means the significant difference between GAPDH and UCCR (P < 0.05), Students t test.