Figure 3From: Comparative transcriptome analysis reveals carbohydrate and lipid metabolism blocks in Brassica napus L. male sterility induced by the chemical hybridization agent monosulfuron ester sodium Strategies for identification of differentially expressed transcripts (DETs) involved in microgametogenesis between the MES-treated and mock-treated plants by two sets of student’s t-test comparisons. (A) Comparisons within groups. The pair-wise comparisons of Student’s t-test between tissues (organs) were carried out within mock-treatment groups and MES-treatment groups, respectively, to detected DETs related to anther development under mock-treatment (control, fertile) and MES-treatment (male sterile) conditions. The criteria for screening DETs were p-value <0.001 and fold change ≥ 2. mock, mock-treatment; MES, MES-treatment; (B) Comparisons between the MES-treated and mock-treated groups. The pair-wise comparisons of Student’s t-test were performed between the corresponding tissues (organs) of the mock-treated group and the MES-treated group to identify DETs related to MES-treatment. The screen criteria were same as above. (C) Venn diagram showing the DETs involved in microgametogenesis between the MES-treated and mock-treated groups. Comparisons within groups produced two sets of DETs, development-related genes in the MES-treated plants and in the mock-treated plants (the left and right cycles). Comparisons between groups also produced two sets of DETs, up-regulated genes and down-regulated genes in MES-treated group (the up and down cycles). These four sets of DETs were all collected, respectively. The common sections (totally 1501 unique DETs, the red and green parts, (2) indicates 2 DETs existing in the both data sets) were considered to be anther development-related genes affected by MES-treatment.Back to article page