Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level

Prielhofer, Roland; Cartwright, Stephanie P; Graf, Alexandra B; Valli, Minoska; Bill, Roslyn M; Mattanovich, Diethard; Gasser, Brigitte

doi:10.1186/s12864-015-1393-8

Table 5 Transcriptional regulation of sugar transporters and sensors

From: Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level

Short name Pp	Description	G-D logFC	G-D adjPV	M-D logFC	M-D adjPV	X-D logFC	X-D adjPV
PpHXT1	P. pastoris major low affinity glucose transporter (major facilitator superfamily)	*−1.31*		*−3.34*	***	*−0.82*	*
ITR2	Myo-inositol transporter	−0.40	*	*−0.88*	*	*−0.62*	***
PAS_c034_0021	Major facilitator superfamily, related to STL1	*−0.59*	**	0.10		−0.55	***
PAS_chr2-1_0006	Major facilitator superfamily, Quinate permease (Quinate transporter) - similar to S. stipitis	−0.06		*−0.80*		−0.01
YBR241C	Putative transporter, member of the sugar porter family	0.12		−0.16		0.26
PpHXT2	P. pastoris putative low affinity glucose transporter of the major facilitator superfamily	−0.10		−0.10		−0.09
STL1-1	Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation in S. cerevisiae	0.08		−0.11		1.23	***
STL1-2	Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation in S. cerevisiae	−0.27		0.40		2.08	***
SNF3	P. pastoris plasma membrane glucose sensor Gss1, regulates glucose transport	0.16		0.44		1.60	***
PAS_chr3_1076	Glycerol proton symporter of the plasma membrane, related to RGT2	0.37		0.65	**	0.62	**
PAS_chr3_1099	Glycerol proton symporter of the plasma membrane, related to STL1 or RGS2	0.34		0.80	**	1.33	***
MAL31	Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter)	0.09		0.81	***	0.68	***
GTH1	P. pastoris major high affinity glucose transporter; similar to K. lactis HGT1	0.17		1.09	***	6.14	***
PpHGT1	P. pastoris high affinity glucose transporter - similar to K. lactis HGT1	0.59		0.86	**	4.91	***
PAS_chr4_0828	Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily	2.35	***	3.65	***	7.30	***
HXK1	Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression in S. cerevisiae is highest during growth on non-glucose carbon sources	0.30		−0.21		1.69	***
HXK2	Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose in S. cerevisiae	−0.12		0.18		0.03
GLK1	Glucokinase; catalyzes the phosphorylation of glucose at C6; expression regulated by non-fermentable carbon sources in S. cerevisiae	*−0.99*	**	*−2.58*	***	−0.34

Log₂ fold changes and adjusted P-values (* adjPV < 0.1; ** adjPV < 0.05; *** adjPV < 0.01) are shown (see Additional file 1: Table S1 for detailed data). Up-regulated genes are in bold letters, down-regulated genes in bold and italics.

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ISSN: 1471-2164

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