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Table 5 Transcriptional regulation of sugar transporters and sensors

From: Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level

Short name Pp Description G-D logFC G-D adjPV M-D logFC M-D adjPV X-D logFC X-D adjPV
PpHXT1 P. pastoris major low affinity glucose transporter (major facilitator superfamily) −1.31   −3.34 *** −0.82 *
ITR2 Myo-inositol transporter −0.40 * −0.88 * −0.62 ***
PAS_c034_0021 Major facilitator superfamily, related to STL1 −0.59 ** 0.10   −0.55 ***
PAS_chr2-1_0006 Major facilitator superfamily, Quinate permease (Quinate transporter) - similar to S. stipitis −0.06   −0.80   −0.01  
YBR241C Putative transporter, member of the sugar porter family 0.12   −0.16   0.26  
PpHXT2 P. pastoris putative low affinity glucose transporter of the major facilitator superfamily −0.10   −0.10   −0.09  
STL1-1 Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation in S. cerevisiae 0.08   −0.11   1.23 ***
STL1-2 Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation in S. cerevisiae −0.27   0.40   2.08 ***
SNF3 P. pastoris plasma membrane glucose sensor Gss1, regulates glucose transport 0.16   0.44   1.60 ***
PAS_chr3_1076 Glycerol proton symporter of the plasma membrane, related to RGT2 0.37   0.65 ** 0.62 **
PAS_chr3_1099 Glycerol proton symporter of the plasma membrane, related to STL1 or RGS2 0.34   0.80 ** 1.33 ***
MAL31 Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter) 0.09   0.81 *** 0.68 ***
GTH1 P. pastoris major high affinity glucose transporter; similar to K. lactis HGT1 0.17   1.09 *** 6.14 ***
PpHGT1 P. pastoris high affinity glucose transporter - similar to K. lactis HGT1 0.59   0.86 ** 4.91 ***
PAS_chr4_0828 Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily 2.35 *** 3.65 *** 7.30 ***
HXK1 Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression in S. cerevisiae is highest during growth on non-glucose carbon sources 0.30   −0.21   1.69 ***
HXK2 Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose in S. cerevisiae −0.12   0.18   0.03  
GLK1 Glucokinase; catalyzes the phosphorylation of glucose at C6; expression regulated by non-fermentable carbon sources in S. cerevisiae −0.99 ** −2.58 *** −0.34  
  1. Log2 fold changes and adjusted P-values (* adjPV < 0.1; ** adjPV < 0.05; *** adjPV < 0.01) are shown (see Additional file 1: Table S1 for detailed data). Up-regulated genes are in bold letters, down-regulated genes in bold and italics.