RT-PCR Analysis of alternative splicing events. RT-PCR validation of three events identified through RNA-seq. (A) Event affected in both mutants simultaneously. (B) Event affected only in prmt5. (C) Event affected only in prmt4a;4b. The read density map for each event evaluated is displayed (WT: black, prmt5: red, prmt4a;4b: blue). A scheme describing each gene is displayed below the read density maps, with exons and introns displayed as boxes and lines respectively. A red square encloses the measured event, while green arrows display the position of the oligo used for the RT-PCR measurement. An image of the agarose gel with the RT- PCR amplicons is displayed next to the read density maps. Black arrows indicate amplicon sizes. +: retrotranscriptase added, −: retrotranscriptase not added, gDNA: genomic DNA control. Each image represents one of three biological replicates measured.