Figure 4From: MicroRNA expression profiles from eggs of different qualities associated with post-ovulatory ageing in rainbow trout (Oncorhynchus mykiss) RT-qPCR validation of differentially expressed miRNAs identified by deep sequencing between D1PO and D14PO eggs. Data were normalized using β-actin and 18S rRNA. The means of the normalized miRNA expression values (n = 4 pools) were calculated and expressed as relative fold changes. Only omy-miR-192a-5p does not match the sequencing data.Back to article page