Figure 1From: Spiked GBS: a unified, open platform for single marker genotyping and whole-genome profiling Primer and amplicon construction. The first round of PCR uses a forward primer containing the M13 sequence to amplify the target region. The second round of PCR extends from the M13 tail and incorporates a unique barcode, leading to a final product containing the sequencer primers, barcode, M13 sequence, and polymorphic target.Back to article page