Skip to main content

Table 1 DNA quantity used for serial dilution library constructions

From: Quality control of microbiota metagenomics by k-mer analysis

  Donor #1 Donor #2
Sample Size (10 x bacteria) Purified dsDNA (ng/ml) 1 DNA for ligation (μg) 2 PCR cycles Purified dsDNA (ng/ml) 1 DNA for ligation (μg) 2 PCR cycles
10 34.9 1.00 6 30.7 1.00 6
9 4.67 0.41 7 7.17 0.35 7
8 2.68 0.07 8 2.63 0.06 8
7 0.358 <0,04 9 0.356 <0,04 9
6 0.296 <0,03 10 0.228 <0,02 10
  1. 1Genomic dsDNA extracted from indicated number of bacteria.
  2. 2Amount of sheared and size purified genomic DNA utilized for ligation with P1 and P2 adaptor oligonucleotides.