Table 1 DNA quantity used for serial dilution library constructions
From: Quality control of microbiota metagenomics by k-mer analysis
Donor #1 | Donor #2 | |||||
|---|---|---|---|---|---|---|
Sample Size (10 x bacteria) | Purified dsDNA (ng/ml) 1 | DNA for ligation (μg) 2 | PCR cycles | Purified dsDNA (ng/ml) 1 | DNA for ligation (μg) 2 | PCR cycles |
10 | 34.9 | 1.00 | 6 | 30.7 | 1.00 | 6 |
9 | 4.67 | 0.41 | 7 | 7.17 | 0.35 | 7 |
8 | 2.68 | 0.07 | 8 | 2.63 | 0.06 | 8 |
7 | 0.358 | <0,04 | 9 | 0.356 | <0,04 | 9 |
6 | 0.296 | <0,03 | 10 | 0.228 | <0,02 | 10 |