Quantitative RT-PCR analysis of
gene expression in
roots treated with MeJA. Fold changes of SmWRKYs in roots of S. miltiorrhiza plantlets treated with MeJA for 12, 24, 36 and 48 h are shown. SmUBQ10 was used as the reference gene. The level of transcripts in roots treated with carrier solution (CK) was arbitrarily set to 1 and the levels in roots treated with MeJA were given relative to this. Mean values and SDs were obtained from three biological and three technical replicates. Y-axis indicates relative expression levels. X-axis indicates different time-points of MeJA treatment. ANOVA (analysis of variance) was calculated using SPSS. P < 0.05 (*) and P < 0.01(**) were considered statistically significant and extremely significant, respectively.