Figure 9

Quantitative RT-PCR analysis of SmWRKY gene expression in S. miltiorrhiza roots treated with MeJA. Fold changes of SmWRKYs in roots of S. miltiorrhiza plantlets treated with MeJA for 12, 24, 36 and 48 h are shown. SmUBQ10 was used as the reference gene. The level of transcripts in roots treated with carrier solution (CK) was arbitrarily set to 1 and the levels in roots treated with MeJA were given relative to this. Mean values and SDs were obtained from three biological and three technical replicates. Y-axis indicates relative expression levels. X-axis indicates different time-points of MeJA treatment. ANOVA (analysis of variance) was calculated using SPSS. P < 0.05 (*) and P < 0.01(**) were considered statistically significant and extremely significant, respectively.