Characterization of three types
. (A) Phylogenetic tree showing relationships among DGAT1, DGAT2, and DGAT3 from Physaria fendleri (Pf), Arabidopsis (At), Brassica napus (Bn), castor bean (Rc), peanut (Ah). PfDGAT1-1 (isotig11157), PfDGAT1-2 (isotig11156), PfDGAT2 (isotig19956), PfDGAT3 (isotig08903), AtDGAT1 (At2g19450), AtDGAT2 (At3g51520), AtDGAT3 (At1g48300), BnDGAT1-1 (AIA77019), BnDGAT1-2 (AIA67020), BnDGAT1-3 (AFM31260), BnDGAT1-4 (AAF64065), RcDGAT1 (XP_002514132), RcDGAT2 (XP_002528531), RcDGAT3 (XP_002519339), AhDGAT3 (AAX62735). The tree was constructed as described in Figure 5B. (B-E) Expression of PfDGAT1-1, PfDGAT1-2, PfDGAT2, and PfDGAT3 in developing seeds and major organs determined by qPCR. Abbreviated names for the genes are described in Figure 3. Each data point represents the mean ± SD of three replicates.