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Figure 4 | BMC Genomics

Figure 4

From: Natural variation of gene models in Drosophila melanogaster

Figure 4

Allelic imbalance of genotype-specific cassette exon inclusion associated with cis -sQTL (a625). Genotype-specific cassette exon that exhibits strong allelic imbalance is shown. The allele-specific exon/intron coverage of genotype-specific splicing event are shown for the F1-hybrids that carried derived (ad) and ancestral (be) variants of cis-sQTL on natural alleles. Tester alleles of all F1-hybrids carried ancestral variant of cis-sQTL. The plots were created separately for the reads expressed from natural (ab) and tester alleles (de). Only genotypes with estimated ns-Ψ and ts-Ψ-values were considered. cf: The distributions of ns-Ψ (c) and ts-Ψ-values (c) for alleles of cis-sQTL. g: RT-PCR validation of gene models. The predicted lengths of PCR-products corresponding to the long and short isoforms are 231 bp and 102 bp, respectively. For other details see the legend to Figure 2.

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