Figure 4
Maximum likelihood gene network of PLA2 genes. Color of the branches indicate function and origin: non-venomous PLA2 genes from non-cnidarian invertebrates (green, B1 [S. purpuratus: sea urchin] and B2 [A. aegypti & C. quinquefasciatus: mosquito; B. floridae: lancelet; C. elegans: nematode; C. intestinalis: tunicate; N. vitripennis: parasitoid wasp; Sy. raphans: sponge; T. adhaerens: placozoan]); PLA2 toxins found in vertebrates (red, C2 [B. asper, B. caudalis, & B. multicinctus: snakes] and C4 [A. eydouxii, C. nigrescens, D. vestigiata, H. stephensii N. scutatus, O. scutellatus, P. porphyriacus, & P. australis: snakes]); non-venomous PLA2 genes found in vertebrates (blue, C1 [T. guttata: zebra finch] C3 [P. major & X. maculatus: fish] and C4 [A. sinensis: alligator; B. taurus: cattle; C. millii: elephant fish; C. lupus familiaris: dog; D. labrax & P. major: fish; E. caballus: horse; G. gallus: chicken; H. glaber, M. musculus, & R. norvegicus: rodents; H. sapien: human; Su. scrofa: pig]) and an invertebrate (green C3 [P. pectinifera: sea star]; and PLA2 genes from sea anemones which may or may not be venomous (black, A1 and A2). Newly-identified candidate toxin genes are in bold with thick branches and the source is indicated (acrorhagi from A: aggressive or NA: non-aggressive polyps). Labels at the terminal tips indicate GenBank accession number and species identity. For full species names refer to S. Table 2. Bootstrap support values greater than 50 are shown.