Table 1 Summary of sequencing, cleanup, and assembly
Sequencing | Aggressive | Non-aggressive |
|---|---|---|
Total reads (% of Lane) | 189,025,968 (46.34 %) | 83,914,158 (19.62 %) |
Trimmomatic cleanup | ||
PE recovered (% of reads) | 90,9451,67 (96.23%) | 41,957,079 (96.33%) |
Forward only (% of reads) | 2,993,563 (3.17%) | 1,257,747 (3.00%) |
Reverse only (% of reads) | 263,778 (0.27%) | 192,526 (0.46%) |
Dropped (% of reads) | 310,476 (0.33%) | 90,639 (0.22%) |
Trinity Assembly | ||
Transcripts | 227,781 | 224,607 |
Components/‘genes’ | 150,270 | 184,129 |
N50 | 1832 | 961 |