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Table 4 Summary of CLARK ’s assignment of 50,646 unigenes (EST assemblies) to barley chromosome arms (assemblies) and centromeres ( k =19)

From: CLARK: fast and accurate classification of metagenomic and genomic sequences using discriminative k-mers

Targets 19-mers Discriminative 19-mers Assignments Low confidence High confidence
1H 180,176,713 108,894,740 8,197 21.1% 78.9%
2HC - 814,357 15 93.3% 6.7%
2HL 103,679,920 64,700,161 4,776 15.8% 84.2%
2HS 90,912,314 54,449,430 3,334 17.3% 82.7%
3HC - 1,532,968 29 79.3% 20.7%
3HL 123,140,951 78,158,244 4,726 16.7% 83.3%
3HS 111,951,787 70,473,478 3,159 20.4% 79.6%
4HC - 3,105,047 54 50.0% 50.0%
4HL 106,999,773 64,749,958 3,531 14.4% 85.6%
4HS 89,027,872 51,612,790 2,468 16.4% 83.6%
5HC - 604,030 9 88.9% 11.1%
5HL 117,915,094 77,128,375 6,111 12.2% 87.8%
5HS 58,067,400 34,037,607 1,619 17.8% 82.2%
6HC - 469,530 9 100.0% 0.0%
6HL 74,485,223 44,221,184 2,973 12.4% 87.6%
6HS 111,834,123 83,957,421 2,721 24.4% 75.6%
7HC - 795,923 9 88.9% 11.1%
7HL 92,603,503 58,159,248 3,556 10.9% 89.1%
7HS 90,217,777 55,276,671 3,350 12.6% 87.4%
Total 1,351,012,450 853,141,162 50,646 16.5% 83.5%
  1. Columns: (1) barley chromosome 1H, twelve chromosome arms, and six centromeres; (2) number of distinct k-mers in each target; (3) number of discriminative k-mers present in target sequences (must occur at least once); (4) number of assigned objects per target; (5) number of low confidence assignment per target; (6) number of high confidence assignment per target; (7) percentage of low confidence assignment (as a fraction of the total number of assigned objects per target); (8) percentage of high confidence assignment (as a fraction of the total number of assigned objects per target).