Table 4 Summary of CLARK ’s assignment of 50,646 unigenes (EST assemblies) to barley chromosome arms (assemblies) and centromeres ( k =19)
Targets | 19-mers | Discriminative 19-mers | Assignments | Low confidence | High confidence |
|---|---|---|---|---|---|
1H | 180,176,713 | 108,894,740 | 8,197 | 21.1% | 78.9% |
2HC | - | 814,357 | 15 | 93.3% | 6.7% |
2HL | 103,679,920 | 64,700,161 | 4,776 | 15.8% | 84.2% |
2HS | 90,912,314 | 54,449,430 | 3,334 | 17.3% | 82.7% |
3HC | - | 1,532,968 | 29 | 79.3% | 20.7% |
3HL | 123,140,951 | 78,158,244 | 4,726 | 16.7% | 83.3% |
3HS | 111,951,787 | 70,473,478 | 3,159 | 20.4% | 79.6% |
4HC | - | 3,105,047 | 54 | 50.0% | 50.0% |
4HL | 106,999,773 | 64,749,958 | 3,531 | 14.4% | 85.6% |
4HS | 89,027,872 | 51,612,790 | 2,468 | 16.4% | 83.6% |
5HC | - | 604,030 | 9 | 88.9% | 11.1% |
5HL | 117,915,094 | 77,128,375 | 6,111 | 12.2% | 87.8% |
5HS | 58,067,400 | 34,037,607 | 1,619 | 17.8% | 82.2% |
6HC | - | 469,530 | 9 | 100.0% | 0.0% |
6HL | 74,485,223 | 44,221,184 | 2,973 | 12.4% | 87.6% |
6HS | 111,834,123 | 83,957,421 | 2,721 | 24.4% | 75.6% |
7HC | - | 795,923 | 9 | 88.9% | 11.1% |
7HL | 92,603,503 | 58,159,248 | 3,556 | 10.9% | 89.1% |
7HS | 90,217,777 | 55,276,671 | 3,350 | 12.6% | 87.4% |
Total | 1,351,012,450 | 853,141,162 | 50,646 | 16.5% | 83.5% |