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Figure 1 | BMC Genomics

Figure 1

From: The dnd operon for DNA phosphorothioation modification system in Escherichia coli is located in diverse genomic islands

Figure 1

Effects of thiourea improved the typeability of Dnd + strains by PFGE. a: PFGE gel obtained by performing PFGE run without the addition of thiourea. b: PFGE gel obtained by performing PFGE run with the addition of thiourea to the agarose gel and running buffer. Lane M: XbaI digested H9812 DNA marker; lanes S1-S7, VTEC isolates from pigs; lanes A1-A3, clinical isolates obtained from medical center A; lanes B1-B2, clinical isolates obtained from medical center B; C, control E. coli isolate which is typeable with and without the addition of thiourea (a and b). Gel images were captured using GelDoc (BioRad, Hercules, CA) digital gel documentation system.

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